Fluorescence of Bacteria, Pollens, and Naturally Occurring Airborne Particles: Excitation/Emission Spectra

Report No. ARL-TR-4722
Authors: Steven C. Hill, Michael W. Mayo, and Richard K. Chang
Date/Pages: February 2009; 62 pages
Abstract: The fluorescence intensity as a function of excitation and emission wavelengths (EEM spectra) was measured for different species of bacteria, biochemical constituents of cells, pollens, and vegetation. This report documents these spectra. This endeavor is part of an effort to determine useful fluorescence excitation and emission wavelengths for discrimination between biological and nonbiological particles, and among different types of biological particles. These results can assist researchers in selecting excitation and emission wavelength bands that allow partial discrimination among particle types. The EEM spectra of dry bacteria samples are different from spectra of bacteria in liquid suspensions. In the dry samples more of the emission tends to occur at longer wavelengths. We discuss appropriate excitation sources for detecting bioaerosols using autofluorescence.
Distribution: Approved for public release
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Last Update / Reviewed: February 1, 2009