Fluorescence Spectra of Individual Flowing Airborne Biological Particles Measured in Real Time

Report No. ARL-TR-1961
Authors: Ronald G. Pinnick, Steven C. Hill, Stanley Niles, Yong-Le Pan, Stephen Holler, Richard K. Chang, Jerold R. Bottiger, Burt V. Bronk, Dominick C. Rozelle, Jay D. Eversole, and Mark Seaver
Date/Pages: February 2001; 29 pages
Abstract: The UV-excited fluorescence spectra of individual flowing biological aerosol particles as small as 2 pm in diameter have been measured in real time (rates up to 10 particles per second). The particles are illuminated with a single shot from a Q-switched 266- or 351-nm laser. The signal-to-noise ratio and resolution of the spectra are sufficient for observing small line-shape differences among various types of bioaerosols (e.g., bacteria versus pollens) and between the same types of bioaerosol prepared under different conditions (e.g., the unstarved and four-month-starved E. coli strain DH5). Multiple-wavelength excitation provides additional information for distinguishing bioaerosols based on their fluorescence spectra.
Distribution: Approved for public release
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Last Update / Reviewed: February 1, 2001