Electrochemical Modification of Glassy Carbon

Report No. ARL-TR-5697
Authors: Amethist S. Finch and James J. Sumner
Date/Pages: September 2011; 22 pages
Abstract: With the sequencing of whole genomes now a reality, the push to synthesize genomes de novo is of great interest. Currently, state-of-the-art oligonucleotide synthetic methods allow for synthesis of up to 200 base pairs. In order to realize increased lengths of an order of magnitude or more, the development of new synthetic strategies and coupling mechanisms is necessary. This project aims to provide an alternative methodology for the synthesis of very long (>200 base pairs) deoxyribonucleic acid (DNA) oligonucleotides. This technique involves using a glass carbon rod as the support for the DNA synthesis and requires development of experimental techniques for irreversibly linking an organic molecule with an aniline-type moiety to the glassy carbon. By in situ conversion of the aniline functional group to its diazonium derivative, we demonstrate direct electrochemical modification of the glassy carbon rod. These preliminary results allowed for the transfer of these materials to collaborators at the Food and Drug Administration for use in their research on alternative methods for automated synthesis of oligonucleotides.
Distribution: Approved for public release
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Last Update / Reviewed: September 1, 2011