Construction of a Cell Based Sensor for the Detection of Autoinducer-2

Report No. ARL-RP-0402
Authors: Matthew D. Servinsky, Patrick C. Allen, Chen-Yu Tsao, Christopher M. Byrd, Christian J. Sund, and William E. Bentley
Date/Pages: September 2012; 14 pages
Abstract: Microbially Induced Corrosion (MIC) occurs at metal surfaces and is associated with microorganisms and their metabolic activities. These microbes can coexist as biofilms, growing as synergistic communities (consortia) that are able to affect electrochemical processes, both cathodic and anodic, often through co-operative metabolism. Recent research has revealed the role of "quorum sensing" molecules in control of microbial activities such as biofilm formation. In this paper, we propose the detection of quorum sensing molecules as a means of detecting bacterial contamination prior to the onset on biofilm formation. Further we outline the development of an E. coli cell based sensor for detection of the quorum sensing molecule Autoinducer-2 (AI-2).
Distribution: Approved for public release
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Last Update / Reviewed: September 1, 2012