Synthesis and Characterization of DNase 1-Stabilized Gold Nanoclusters

Report No. ARL-TR-7129
Authors: Abby L West ; Mark H Griep; Daniel P Cole; Shashi P Karna
Date/Pages: October 2014; 26 pages
Abstract: DNase 1-stabilized gold nanoclusters (DNase 1 AuNCs) were synthesized with multiple energy levels in a metal-concentration-dependant fashion. From multiple characterization techniques including fluorescence spectroscopy, high-resolution transmission electron microscopy and X-ray photoelectron spectroscopy, we assigned 2 reaction products to contain pure populations of clusters with core sizes of 8 and 25 Au atoms for the products where 10- and 2.5-mM HAuCl4 was used in the reaction, respectively, and 2 reaction products to contain either mixtures of the Au8 and Au25 clusters or Au nanoparticles (AuNPs) dependent upon the initial Au concentration. An initial HAuCl4 concentration of 5 mM produced a small amount of AuNPs but no NCs, while an initial concentration of 0.5-mM HAuCl4 resulted in a mixture of Au8 and Au25 clusters with a higher percentage of Au25 clusters. The DNase 1 Au8NCs exhibiting blue fluorescence have an excitation and emission maxima of 390 and 460 nm, respectively, whereas the DNase 1 Au25NCs are red-emitting and have an excitation and emission maxima of 490 and 640 nm, respectively.
Distribution: Approved for public release
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Last Update / Reviewed: October 1, 2014